Concurrent unilateral chromatid damage and DNA strand breakage in response to 6-thioguanine treatment

Concurrent unilateral chromatid damage and DNA strand breakage in response to 6-thioguanine treatment.

The delayed cytotoxicity of 6-thioguanine (TG) may relate to the arrest of cells in G2 upon completion of one cell cycle after drug exposure. In Chinese hamster ovary (CHO) cells, both the unilateral chromatid damage in G2 chromosomes, determined by induction of premature condensed chromosome condensation [Maybaum and Mandel, Cancer Res. 43, 3852 (1983)], and incorporation of TG into DNA result...

FBH1 promotes DNA double-strand breakage and apoptosis in response to DNA replication stress

Proper resolution of stalled replication forks is essential for genome stability. Purification of FBH1, a UvrD DNA helicase, identified a physical interaction with replication protein A (RPA), the major cellular single-stranded DNA (ssDNA)-binding protein complex. Compared with control cells, FBH1-depleted cells responded to replication stress with considerably fewer double-strand breaks (DSBs)...

Bleomycin-induced alkaline-labile damage and direct strand breakage of PM2 DNA.

Bleomycin-induced breakage of an isolated covalently closed circular DMA from bacteriophage PM2 was as sayed fluorometrically after agarose gel electrophoresis and staining with ethidium bromide. When bleomycindamaged DMA was assayed under neutral conditions, there was a decrease in the amount of Form I DMA and a simultaneous increase in both Forms II and III of DMA. However, when the damage wa...

Nijmegen breakage syndrome: clinical manifestation of defective response to DNA double-strand breaks.

Nijmegen breakage syndrome is a rare autosomal recessive genetic disease belonging to a group of disorders often called chromosome instability syndromes. In addition to a characteristic facial appearance and microcephaly, patients suffering from Nijmegen breakage syndrome have a range of symptoms including radiosensitivity, immunodeficiency, increased cancer risk and growth retardation. The und...

Oxidative DNA Damage and Pro-inflammatory Response In Chronic Exposure To Cement Dust